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Rabbit Anti-FAK

时间:2012-8-3阅读:473
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Rabbit Anti-FAK
FROM: Abcam
Cat. Number:  Rs-1340R
Quantity size:  0.1ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.
 
Specificity:      ·anti-FAK is a rabbit monoclonal antibody unconjugated
                ·specific for FAK of  hu
                ·epitope mapping near the amino terminus of FAK of Hu
                · use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
 
Rabbit Anti-FAK
Application:     · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rabbit Anti-FAK

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