Human Vitamin D(VD)FOR RESEARCH USE ONLY
1 | wash solution | 20ml×1bottle | 7 | Stopp Solution | 6ml×1 bottle |
2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Standard(1600 nmol/L) | 0.5ml×1 bottle |
3 | Microelisa stripplate | 12well×8strips | 9 | Standard diluent | 1.5ml×1bottle |
4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
6 | Chromogen Solution B | 6ml×1 bottle | 12 | Sealed bags | 1 |
800 nmol/L | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
400 nmol/L | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
200 nmol/L | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
100 nmol/L | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
50 nmol/L | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
Standard, Sample diluent |
AddStandard, Sample diluent, incubate for 30 min at 37℃. |
Wash 5 time,AddHRP-Conjugate reagent, incubate for 30 min at 37℃. |
Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃. |
AddStopp Solution |
Read absorbance at 450nm within 15 min |
calculate |
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