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Anti-MMP-13 抗体说明书

时间:2012-2-20阅读:557
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 Anti-MMP-13 抗体说明书

Quantity size :   200ul (dilute with pH 7.4    0.01 M PBS or antibody diluent )
 
Background: Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.
 

 Anti-MMP-13 抗体说明书

pecificity :   
        Anti MMP13 is a goat polyclonal antibody unconjugated                
         specific for MMP13 of rabbit, mouse and rat           
         use for western blotting,elisa,immunoprecipitation and immunohistochemistry
         Protein G affinity chromatography purification, purity :>95%
         Isotype:  IgG
         mol wt:  38 kDa
 
Application :  
        Western blotting 1:100-500                
         Immunohistochemistry 1:100-500                 
         ELISA 1:500-1000                  
         Optimal working dilutions must be determined by the end user.
 
Storage: Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4
 
 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-MMP-13 抗体说明书

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