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Anti-IGF1R/CD221 抗体说明书

时间:2012-2-22阅读:407
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 Anti-IGF1R/CD221 抗体说明书

Cat. Number:  Rs-0227R
Quantity size:  0.1ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
 

 Anti-IGF1R/CD221 抗体说明书

Specificity:      ·anti- CD221 is a rabbit monoclonal antibody unconjugated
                ·specific for CD221 of  hu, rat and mo 
                ·epitope mapping near the amino terminus of CD221 of  
                   hu, rat and mo origin
                · use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
 
Application:     · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-IGF1R/CD221 抗体说明书

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