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Anti-Integrin αVβ3抗体说明书

时间:2012-3-12阅读:1024
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 Anti-Integrin αVβ3抗体说明书

Quantity size:  0.1ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background: Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in plaets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.

 Anti-Integrin αVβ3抗体说明书

 
Specificity:       ·anti- Integrin αVβ3 is a rabbit monoclonal antibody unconjugated
                ·specific for Integrin αVβ3 of human  
                ·epitope mapping near the amino terminus of Integrin αVβ3 of  
                   rat origin
                · use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
 
Application:     · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-Integrin αVβ3抗体说明书

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