Mouse Anti-TSHR抗体说明书
Catalog Number:Rs-1380R
Quantity size:0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: The glycoprotein hormone receptor family consists of the luteinizing hormone receptor, the follicle-stimulating hormone receptor, and the thyroid stimulating hormone（TSH） receptor. TSH, which is released from the pituitary gland, binds to the TSH receptor on thyroid cells to control size and function of the thyroid gland （De Felice et al. 2004）. The TSH receptor signals through Gs to elevate intracellular cAMP in the thyroid gland, which regulates iodide uptake, and transcription of thyroglobulin （Tg）, thyroid peroxidase （TPO）, and sodium-iodide symporter. The TSH receptor also signals Gq and phospholipase C to regulat iodide efflux, H2O2 production, and thyroglobulin iodination. Autoimmunity to the TSH receptor causes hyperthyroidism （Graves disease） or hypothyroidism （Hashimoto thyroiditis） when the autoantibodies function as agonists or antagonists, respectively, at the TSH receptor （Rapoport and McLachlan, 2001; Davies et al., 2002）. Millipore’s cloned human TSH receptor-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant TSH receptor expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TSH and its ligands
Mouse Anti-TSHR抗体说明书
Specificity : ·anti-TSHR is a Mouse monoclonal antibody unconjugated
·specific for TSHR of Human,rat
·use for western blot, elisa, immunoprecipitation
and immunohistochemistry
·affinity chromatography purification, purity :>95%
·isotype: IgG
·mol wt: 38kDa
Application :· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Western blotting 1:100-500
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti-TSHR抗体说明书
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