猪胰岛素(INS)ELISA试剂盒
Porcine Insulin(INS)ELISA Kit
实验原理：
本试剂盒仅供研究使用，用于测定猪血清，血浆及相关液体样本中胰岛素(INS)的含量。应用双抗体夹心法测定标本中猪胰岛素(INS)水平。用纯化的猪胰岛素(INS)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胰岛素(INS)，再与HRP标记的胰岛素(INS)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的胰岛素(INS)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中猪胰岛素(INS)浓度。
Principle of the assay
The kit assay Porcine INS level in the sample，use Purified Porcine INS to coat microtiter plate wells, make solid-phase antibody, then add INS to wells, Combined INS which With HRP labeled ， become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司
