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技术文章

Rabbit Anti-BMP2 protein, FITC conjugated

阅读:1164发布时间:2010-12-8

Quantity size : 0.2ml (2mg/ml, Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Sodium azide. Reconstitute with 0.2ml sterile distilled water. )
Background: BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. Also known as: bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2; BMP2A.
Specificity :   · anti- BMP2 is a rabbit polyclonal antibody, FITC conjugated
                         · specific for BMP2 of human, mouse, rat, chi, dog, sheep and bov 
                         · epitope mapping near the C- terminus of BMP2 of human, mouse, rat, chi, heep and bovine
                         · human, mouse, rat, chi, dog, sheep and bovine cross reacted 
                         · Protein G affinity chromatography purification, purity :>95%
                         · Isotype: IgG
                         · mol wt: 13; 4kDa
Application:    · FCM: 1:20-100
                        · Immunohistochemistry: 1:20-200 
                        · Immunocytochemistry: 1:20-1:200 
                        · Excitation spectrum: 490-495nm
                        · Emission spectrum: 520-530nm 
                        · Optimal working dilutions must be determined by the end user. 
                        · protect from light.
Storage: Store at –20 º for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20º. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 º.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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