女人天天干夜夜爽视频 荳頑ｵｷ騾ｸ蟲ｰ逕溽黄遘第橿譛蛾剞蜈ｬ蜿ｸ

蠖灘燕菴咲ｽｮ뮌郌a href="/st44507/">女人天天干夜夜爽视频 荳頑ｵｷ騾ｸ蟲ｰ逕溽黄遘第橿譛蛾剞蜈ｬ蜿ｸ>>謚€譛ｯ譁・ｫ>>螟ｧ鮠扈蜢Ȧ髣ｴ邊倬刋蛻鰭ｭ鰭뮌鰭CAM-1/CD54뮌右LISA譽€豬玖ｯ募奄逶賌/a>

螟ｧ鮠扈蜢Ȧ髣ｴ邊倬刋蛻鰭ｭ鰭뮌鰭CAM-1/CD54뮌右LISA譽€豬玖ｯ募奄逶賌/h1>
譌ｶ髣ｴ뮌Ȧ014-4-24髦蚢ｯｻ뮌郈56
蛻蜠ｺｫ뮌鰭
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荳頑ｵｷ騾ｸ譎礼函迚ｩ遘第橿譛蛾剞蜈ｬ蜿ｸ莉｣逅蜠ｸ榊酔蜩∫煙莉ｷ譬ｼ譯｣谺｡逧ЕLISA隸募奄逶偵€よ蒲菴謎ｺｧ蜩∫ｭ衄 蜩∫ｧ榊､夲ｼ瑚ｴｨ驥丞･ｽ뮌御ｻｷ譬ｼ螳樊Β뮌悟ｹｶ荳碑ｿ俶署萓帛鰭雍ｹ莉｣譽€豬区恪蜉｡縲蓌/span>
譛ｬ蜈ｬ蜿ｸ逧Ȧ峩螟壻ｺｧ蜩・ｼ瑚ｯｷ轤ｹ蜃ｻ蜈ｬ蜿ｸ뮌喇ttp://www.yihanbio.com/

譛ｬ隸募奄逶貞宵閭ｽ逕ｨ莠守ｧ大ｭｦ遐皮ｩｶ뮌御ｸ榊ｾ礼畑莠主現蟄ｦ隸頑妙

螟ｧ鮠扈蜢Ȧ髣ｴ邊倬刋蛻鰭ｭ鰭뮌鰭CAM-1/CD54뮌右LISA譽€豬玖ｯ募奄逶賌/span>

菴ｿ逕ｨ隸ｴ譏惹ｹｦ

譽€豬句次逅蛌/span>

隸募奄逶帝㊦逕ｨ蜿梧蒲菴謎ｸ€豁･螟ｹ蠢Ȧｳ暮Ȧ閨泌鰭逍ｫ蜷ｸ髯蘢ｯ暮ｪ鯉ｼ鰭LISA뮌峨€ょｾ€鬚鰭虲蛹蚢｢ｫ螟ｧ鮠扈蜢Ȧ髣ｴ邊倬刋蛻鰭ｭ鰭뮌鰭CAM-1/CD54뮌画黒闔ｷ謚嶺ｽ鍋噪蛹蚢｢ｫ蠕ｮ蟄比ｸｭ뮌御ｾ晄ｬ｡蜉蜈･譬Ȧ悽縲∵鰭㊥蜩√€？RP譬螢ｮｰ逧Ȧ｣€豬区蒲菴難ｼ檎ｻ剰ｿȦｸｩ閧ｲ蟷ｶ*豢玲ｶ､縲ら畑蠎慕黄TMB譏ｾ濶ｲ뮌卦MB蝨ｨ霑Ȧｰｧ蛹也黄驟ｶ逧鰭ぎ蛹紋ｸ玖ｽｬ蛹匁蟶闢晁牡뮌悟ｹｶ蝨ｨ驟ｸ逧蘠ｽ懃畑荳玖ｽｬ蛹匁蟶zui扈育噪鮟蘢牡縲る｢懆牡逧Ȧｷｱ豬鰭柱譬ｷ蜩∽ｸｭ逧鰭､ｧ鮠扈蜢Ȧ髣ｴ邊倬刋蛻鰭ｭ鰭뮌鰭CAM-1/CD54뮌牙争豁｣逶ｸ蜈ｳ縲ら畑驟ｶ譬螠ｻｪ蝨ｨ450nm 豕｢髟ｿ荳区ｵ句ｮ郌/span>蜷ｸ蜈牙ｺｦ뮌・D 蛟ｼ뮌会ｼ瑚ｮ｡邂玲ｷ蜩∵ｵ灘ｺｦ縲蓌/span>

譬ｷ蜩∵噺髮・€∝､・炊蜿贋ｿ晏ｭ俶婿豕蹌/span>

1.  陦€貂・ｼ壻ｽｿ逕ｨ荳榊性辜ｭ蜴溷柱蜀Ȧｯ堤ｴ逧蘢ｯ慕ｮ｡뮌梧桃菴懆ｿ・ｨ倶ｸｭ驕ｿ蜈堺ｻｻ菴慕ｻ蜢Ȧ蛻ｺ豼€뮌梧噺髮蜢｡€豸ｲ蜷趣ｼ・000霓ｬ遖ｻ蠢鰭0蛻鰭帖蟆蜢｡€貂鰭柱郤｢扈蜢Ȧ霑鰭€溷ｰ丞ｿ鰭慍蛻・ｦｻ縲蓌/span>

2.  陦€豬・ｼ哘DTA縲∵泛讙ｬ驟ｸ逶先Ȧ閧晉ｴ謚怜鰭縲・000霓ｬ遖ｻ蠢・0蛻鰭帖蜿紋ｸ頑ｸ・€蓌/span>

3.  扈蜢Ȧ荳頑ｸȦｶｲ뮌・000霓ｬ遖ｻ蠢鰭0蛻鰭帖蜴ｻ髯､鬚礼ｲ貞柱閨壼粋迚ｩ縲蓌/span>

4.  扈・ｻ鰭劇豬・ｼ壼ｰ・ｻ・ｻ鰭刈蜈･騾る㍼逕溽炊逶先ｰｴ謐｣遒弱€・000霓ｬ遖ｻ蠢鰭0蛻鰭帖蜿紋ｸ頑ｸ・€蓌/span>

5.  菫晏ｭ假ｼ壼ｦよ棡譬ｷ譛ｬ謾ｶ髮鰭錘荳榊所譌ｶ譽€豬具ｼ瑚ｯｷ謖我ｸ€谺｡逕ｨ驥丞Ȧ陬・ｼ悟・蟄倅ｺ鰭20邃・ｼ碁∩蜈榊渚螟榊・陞搾ｼ悟惠螳､貂ｩ荳玖ｧ｣蜀ｻ蟷ｶ遑ｮ菫晄ｷ蜩∝插蛹€蝨ｰ蜈鰭Ȧ隗｣蜀ｻ縲蓌/span>

閾ｪ螟・黄蜩葌/span>

  1. 驟ｶ譬螠ｻｪ뮌蟈50nm뮌行/span>
  2. 鬮倡ｲｾ蠎ｦ蜉譬ｷ蝎ｨ蜿頑棯螟ｴ뮌郆.5-10uL縲Ȧ-20uL縲Ȧ0-200uL縲Ȧ00-1000uL
  3. 37邃蕌/span>諱呈ｸｩ邂ｱ

謫堺ｽ懈ｳｨ諢丈ｺ矩｡ｹ

  1.   隸募奄逶剃ｿ晏ｭ伜惠2-8邃・ｼ御ｽｿ逕ｨ蜑榊ｮ､貂ｩ蟷ｳ陦｡20蛻鰭帖縲ゆｻ主蜆邂ｱ蜿門Ȧ逧Ȧｵ鍋ｼｩ豢玲ｶ､豸ｲ莨壽怏扈捺匕뮌瑚ｿ吝ｱ樔ｺ取ｭ｣蟶ｸ邇ｰ雎｡뮌梧ｰｴ豬ｴ蜉辜ｭ菴ｿ扈捺匕*貅ｶ隗｣蜷主鰭菴ｿ逕ｨ縲蓌/span>
  2.   螳樣ｪ御ｸｭ荳咲畑逧Ȧ攸譚｡蠎皮ｫ句叉謾ｾ蝗櫁Ȧ蟆∬｢倶ｸｭ뮌悟ｯ鰭ｰ・ｼ井ｽ取ｸｩ蟷ｲ辯･뮌我ｿ晏ｭ倥€蓌/span>
  3.   鬚鰭､・炊蜷守噪譬ｷ譛ｬ譌髴€遞€驥奇ｼ檎峩謗･蜿鰭0μL蜉譬ｷ蜊ｳ蜿ｯ縲蓌/span>
  4.   荳･譬ｼ謖臥・隸ｴ譏惹ｹｦ荳ｭ譬ȦȦ逧Ȧ慮髣ｴ縲∝刈豸ｲ驥丞所鬘ｺ蠎剰ｿ幄｡梧ｸｩ閧ｲ謫堺ｽ懊€蓌/span>
  5.  謇€譛画ｶｲ菴鍋ｻ鰭Ȧ菴ｿ逕ｨ蜑榊鰭蛻Ȧ燥蛹€縲蓌/span>

隸募奄逶堤ｻȦ蟶

蟄秘鰭鄂ｮ

蟄秘鰭鄂ｮ

蟄Ȧtimes;8譚｡

蟄Ȧtimes;4譚｡

뮌行/span>

×豢玲ｶ､郛灘Ȧ豸ｲ

蠑

蠑

莉ｽ

莉ｽ

荳ｪ

荳ｪ

縲葌/span>24縲葌/span>6縲葌/span>1.5 pg/mL.

隸募奄逧鰭㊥螟蝌/span>

 20×豢玲ｶ､郛灘Ȧ豸ｲ逧・ｨ€驥奇ｼ夊頂鬥乗ｰｴ謖鰭뮌Ȧ0遞€驥奇ｼ悟叉1莉ｽ逧Ȧ0×豢玲ｶ､郛灘Ȧ豸ｲ蜉19莉ｽ逧蘢頂鬥乗ｰｴ縲蓌/span>

豢玲攸譁ｹ豕蹌/span>

  1.   謇句ｷ･豢玲攸뮌夂畠蟆ｽ蟄泌鰭豸ｲ菴難ｼ梧ｯ丞ｭ泌刈貊｡豢玲ｶ､豸ｲ뮌碁撕鄂ｮ1min蜷守畠蟆ｽ蟄泌鰭豸ｲ菴難ｼ悟惠蜷ｸ豌ｴ郤ｸ荳頑牛蟷ｲ뮌悟ｦよｭ､豢玲攸5谺｡縲蓌/span>
  2.   閾ｪ蜉ｨ豢玲攸譛ｺ뮌壽ｯ丞ｭ疲ｳｨ蜈･豢玲ｶｲ350μL뮌梧ｵｸ豕｡1min뮌梧ｴ玲攸5谺｡縲蓌/span>

謫堺ｽ懈ｭ･鬪､

  1.   莉主ｮ､貂ｩ蟷ｳ陦｡20min蜷守噪體晉ｮ碑｢倶ｸｭ蜿門Ȧ謇€髴€譚ｿ譚｡뮌悟黄菴呎攸譚｡逕ｨ閾ｪ蟆∬｢句ｯ鰭ｰ∵叛蝗鋈邃・€蓌/span>
  2.   隶ｾ鄂ｮ譬鰭㊥蜩∝ｭ斐€∵ｷ譛ｬ蟄泌柱遨ｺ逋ｽ蟄費ｼ觌strong>遨ｺ逋ｽ蟄比ｻ€荵磯鰭荳榊刈뮌帶鰭㊥蜩∝ｭ泌推蜉荳榊酔豬灘ｺｦ逧Ȧ鰭㊥蜩鰭0μL뮌酌/span>
  3.   蠕Ȧｵ区ｷ譛ｬ蟄泌虲蜉蠕Ȧｵ区ｷ譛ｬ10μL뮌悟鰭蜉*40μL뮌酌/span>
  4.   髫丞錘譬鰭㊥蜩∝ｭ泌柱譬ｷ譛ｬ蟄比ｸｭ뮌育ｩｺ逋ｽ蟄比ｸ榊刈뮌行/strong>蜉蜈･霎｣譬ｹ霑Ȧｰｧ蛹也黄驟ｶ뮌蟒RP뮌画螢ｮｰ逧Ȧ｣€豬区蒲菴鰭00μL뮌鯉ｼ檎畑蟆∵攸閹懷ｰ∽ｽ丞渚蠎泌ｭ費ｼ・7邃Ȧｰｴ豬ｴ髞ȦȦ諱呈ｸｩ邂ｱ貂ｩ閧ｲ60min縲蓌/span>
  5.   蠑鰭悉豸ｲ菴難ｼ悟精豌ｴ郤ｸ荳頑牛蟷ｲ뮌梧ｯ丞ｭ泌刈貊｡豢玲ｶ､豸ｲ뮌碁撕鄂ｮ1min뮌檎畠蜴ｻ豢玲ｶ､豸ｲ뮌悟精豌ｴ郤ｸ荳頑牛蟷ｲ뮌悟ｦよｭ､驥榊､肴ｴ玲攸5谺｡뮌井ｹ溷庄逕ｨ豢玲攸譛ｺ豢玲攸뮌峨€蓌/span>
  6.   謇€譛牙ｭ泌刈蜈･蠎慕黄A縲。蜷0μL뮌・7邃鰭∩蜈牙ｭｵ閧ｲ15min縲蓌/span>
  7.   謇€譛牙ｭ泌刈蜈･扈域ｭ｢豸ｲ50μL뮌鰭5min蜀・ｼ悟惠450nm豕｢髟ｿ螟Ȧｵ句ｮ壼推蟄皮噪OD蛟ｼ縲蓌/span>

扈捺棡蛻､譁ｭ

 扈伜宛譬鰭㊥譖ｲ郤ｿ뮌壼惠Excel蟾･菴懆｡ｨ荳ｭ뮌御ｻ･譬鰭㊥蜩∵ｵ灘ｺｦ菴懈ｨｪ蝮先・ｼ悟ｯｹ蠎念D蛟ｼ菴懃ｺｵ蝮先・ｼ檎ｻ伜宛蜃ｺ譬鰭㊥蜩∫ｺｿ諤ｧ蝗槫ｽ呈峇郤ｿ뮌梧潔譖ｲ郤ｿ譁ｹ遞玖ｮ｡邂怜推譬ｷ譛ｬ豬灘ｺｦ蛟ｼ縲蓌/span>

隸募奄逶呈€ｧ閭ｽ

1.  蜃・｡ｮ諤ｧ뮌壽鰭㊥蜩∫ｺｿ諤ｧ蝗槫ｽ剃ｸ朱｢Ȧ悄豬灘ｺｦ逶ｸ蜈ｳ邉ｻ謨ｰR蛟ｼ뮌悟､ｧ莠守ｭ我ｺ諆.9900縲蓌/span>

2.  轣ｵ謨丞ｺｦ뮌營ui菴取｣€豬区ｵ灘ｺｦ蟆丈ｺ鰭.0 pg/ml縲蓌/span>

3.  迚ｹ蠑よ€ｧ뮌壻ｸ堺ｸ主Ȧ螳鰭庄貅ｶ諤ｧ扈捺桷邀ｻ莨ｼ迚ｩ莠､蜿牙渚蠎斐€蓌/span>

4.  驥榊､肴€ｧ뮌壽攸蜀・€∵攸髣ｴ蜿伜ｼらｳｻ謨ｰ蝮鰭ｰ丈ｺ鰭5%縲蓌/span>

5.  雍ｮ阯擾ｼȦ-8邃・ｼ碁∩蜈蛾亟貎ｮ菫晏ｭ倥€蓌/span>

6.  譛画譜譛滂ｼȦ荳ｪ譛蟌/span>

蜈崎ｴ｣螢ｰ譏諌/span>

1.   隸募奄逶剃ｻ蚠ｾ帷皮ｩｶ菴ｿ逕ｨ뮌御ｸ榊ｾ礼畑莠惹ｸｴ蠎雁ｮ樣ｪ梧Ȧ莠ｺ菴灘ｮ樣ｪ鯉ｼ悟凄蛻呎園莠ｧ逕溽噪荳€蛻鰭錘譫懶ｼ檎罰螳樣ｪ瑚€Ȧ価諡・ｼ梧悽蜈ｬ蜿ｸ讎ゆｸ崎ｴ溯ｴ｣縲蓌/span>

2.   荳･譬ｼ謖臥・隸ｴ譏惹ｹｦ謫堺ｽ懶ｼ悟ｮ樣ｪ瑚€蚢ｿ晏渚隸ｴ譏惹ｹｦ謫堺ｽ懶ｼ悟錘譫懃罰螳樣ｪ瑚€Ȧ価諡・€蓌/span>

fa・ｽ・ｽ-: ・ｽ・ｽ・ｽ・ｽ・ｽ菴・mso-hansi-font-family:譁ｹ豁｣蟋壻ｽȦ#39;>荳･譬ｼ謖臥・隸ｴ譏惹ｹｦ謫堺ｽ懶ｼ悟ｮ樣ｪ瑚€蚢ｿ晏渚隸ｴ譏惹ｹｦ謫堺ｽ懶ｼ悟錘譫懃罰螳樣ｪ瑚€Ȧ価諡・€蓌/p>

 

es i・ｽ-Rm・ｽ・ｽ・ｽ・ｽ・ｽ・ｽ・ｽ蜷取婿蜿ｯ菴ｿ逕ｨ뮌碁Ȧ譬鰭桁陲ｫ譚ｿ蠑€蟆∝錘螯よ悴逕ｨ螳鯉ｼ梧攸譚｡蠎碑｣鰭鰭蟇鰭ｰ∬｢倶ｸｭ菫晏ｭ倥€蓌/p>

 

  1. 蜷Ȧｭ･蜉譬ｷ蝮鰭ｺ比ｽｿ逕ｨ蜉譬ｷ蝎ｨ뮌悟ｹｶ扈丞ｸｸ譬｡蟇ｹ蜈ｶ蜃・｡ｮ諤ｧ뮌御ｻ･驕ｿ蜈崎ｯ暮ｪ瑚ｯｯ蟾ｮ縲ゆｸ€谺｡蜉譬ｷ譌ｶ髣ｴ謗ｧ蛻ｶ蝨ｨ5隸ｷ豈乗ｬ｡豬句ｮ夂噪蜷梧慮蛛壽鰭㊥譖ｲ郤ｿ뮌悟★螟榊ｭ斐€ょｦよȦ悽荳ｭ蠕Ȧｵ狗黄雍ｨ蜷ｫ驥剰ｿ鰭ｫ假ｼ域ｷ譛ｬOD蛟ｼ뮌会ｼ瑚ｯｷ蜈育畑譬ｷ蜩∫ｨ€驥頑ｶｲ遞€驥贋ｸ€螳壼€肴焚뮌蟌/span>n×n×5蟆∵攸閹懷宵髯蝉ｸ€谺｡諤ｧ菴ｿ逕ｨ뮌御ｻ･驕ｿ蜈堺ｺ､蜿画ｱ｡譟薙€蓌/span>
  2. 荳･譬ｼ謖臥・隸ｴ譏惹ｹｦ逧Ȧ桃菴懆ｿ幄｡鯉ｼ瑚ｯ暮ｪ檎ｻ捺棡蛻､螳壼ｿ鰭｡ｻ莉･驟ｶ譬螠ｻｪ隸ｻ謨ｰ荳ｺ蜃蛌/span>.
  3. 譛ｬ隸募奄荳榊酔謇ｹ蜿ｷ扈鰭Ȧ荳榊ｾ玲ｷｷ逕ｨ縲蓌/span>

10. 隶｡邂暦ｼ郌/span>

蛟ｼ荳ｺ郤ｵ蝮先・ｼ觌/span>   

蛟ｼ逕ｱ譬鰭㊥譖ｲ郤ｿ譟･蜃ｺ逶ｸ蠎皮噪豬灘ｺｦ뮌帛鰭荵倅ｻ･遞€驥裌/span>      

蛟ｼ隶｡邂怜Ȧ譬蝌/span>      

蛟ｼ      

蛟肴焚뮌悟叉荳ｺ譬ｷ蜩∫噪螳樣刔豬灘ｺｦ縲蓌/span>                  

 

                                             

 

隸募奄逶呈€ｧ閭ｽ뮌郌/span>

1.蛟ｼ荳ｺ0.990謇ｹ蜀蚠ｸ取音隗∝ｺ泌Ȧ蛻ｫ蟆丈ｺ諌/span>9%譽€豬玖激蝗ｴ뮌郌/span>                                             

0.5μg/L -15μg/L                                     

                           

隸募奄逶剃ｿ晏ｭ假ｼ郌/span>邃蕌/span>뮌取怏謨域悄뮌郌/span>6