女人天天干夜夜爽视频 *化01群稻叶型霍乱弧菌抗体IgG相关知识

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女人天天干夜夜爽视频 *化01群稻叶型霍乱弧菌抗体IgG相关知识 Anti-V.cholera Ogawa/Biotin  *化01群稻叶型霍乱弧菌抗体IgG

Anti-V.cholera Ogawa/Biotin  *化01群小川型霍乱弧菌抗体IgG

Anti-Influenza A Virus H1/HRP  辣根过氧化物酶标记羊抗季节性A型流感病毒H1抗体IgG

Anti-Influenza A Virus H3/HRP  辣根过氧化物酶标记羊抗季节性流感病毒H3抗体IgG

Anti-H1N1-A/HRP（Influenza A virus）California  辣根过氧化物酶标记兔抗季节性A型人流感病毒H1N1抗体IgG（美国加州型）

Anti-CD4/Biotin  *化CD4抗体IgG

Anti-VDAC/FITC  荧光素标记电压依赖性阴离子通道抗体IgG

Anti-CD14/Biotin  *化CD14抗体IgG

Anti-VEGF/FITC  荧光素标记VEGF抗体IgG

Anti-VEGF（Rabbit）/FITC  荧光素标记血管内皮生长因子抗体IgG

Anti-VEGF-A/FITC  荧光素标记血管内皮生长因子A抗体IgG

Anti-VEGF-B/FITC  荧光素标记血管内皮生长因子B抗体IgG

Anti-VEGF-C/FITC  荧光素标记血管内皮生长因子C型抗体IgG

Anti-VEGF/RBITC  罗丹明标记VEGF抗体IgG

Mouse Anti-human VEGF/FITC  荧光素标记小鼠抗人VEGF单克隆抗体IgG

Anti-VEGFR1/VEGF-R1/Flt-1 /FITC  荧光素标记血管内皮生长因子受体1抗体IgG

Anti-VEGFR2（VEGF-R2）Flk1/FITC  荧光素标记血管内皮生长因子受体2抗体IgG

Anti-SV2A/FITC  荧光素标记突触泡蛋白2A抗体IgG

Anti-sVEGFR2/sFLK-1/FITC  荧光素标记可溶性血管内皮生长因子受体2抗体（人）IgG

Anti-VEGFR-3/FLt-4 /FITC  荧光素标记血管内皮细胞生长因子受体-3IgG

Anti-HSP-70/RBITC  罗丹明标记热休克蛋白-70抗体IgG

Anti-VHL/FITC  荧光素标记一种肿瘤抑制因子抗体IgG

Anti-Vimentin/FITC  荧光素标记波形蛋白抗体IgG

Anti-VIP/FITC  荧光素标记血管活性肠肽抗体蛋白抗体IgG

Anti-Visfatin-1/PBEF1（NT）/FITC  荧光素标记内脂素/内脏脂肪素/腹脂素/内肥素 抗体IgG

Anti-Visfatin-1/PBEF1（NT） hu、 mo、 rat、MK /FITC  荧光素标记内脂素/内脏脂肪素（N端抗体）IgG

Anti-Visfatin-1/PBEF1（NT） Human /FITC  荧光素标记内脂素/内脏脂肪素（N端抗体）IgG

Anti-Visfatin-2（CT）/FITC  荧光素标记内脂素/内脏脂肪素（C端抗体）IgG

Anti-VPS18/FITC  荧光素标记空泡分选蛋白18抗体IgG

Anti-VR1/FITC  荧光素标记辣椒素受体抗体IgG

Anti-VSIG4 /FITC  荧光素标记T淋巴细胞负调节蛋白之一抗体IgG

Anti-VSV-G tag/FITC  荧光素标记VSV-G tag标签抗体IgG

Anti-VSV-G tag/HRP  辣根过氧化物酶标记VSV-G tag标签抗体IgG

Anti-VTG/FITC  荧光素标记卵黄蛋白原抗体IgG

Agarose plug:
1% agarose dissolved in 1x Resolving gel buffer.
（I make 50 ml, keep melting it as I need it, and re-adding water to maintain agarose conc.）
Resolving gel: 24 ml of a 9% gel
5.4 ml 40% acrylamide/bisacrylamide （29:1 mix）
3 ml 8x Resolving gel buffer
15.6 ml water
12 µl TEMED
60 µl 20% ammonium persulfate
Stacking gel: 8 ml
1 ml 40% acrylamide/bisacrylamide （29:1 mix）
2 ml 4x Stacking gel buffer
5 ml water
8 µl TEMED
21.6 µl 20% ammonium persulfate

C. Preparation of gel

Assemble the glass plates and spacers （1.5 mm thick）.
Pour an agarose plug （1-2 mm）.
Pour the running gel to about 1 cm below the wells of the comb （~20 ml）.
Seal with 1 ml water-saturated 1-butanol.
（Can stop here and leave gel as is overnight if you want.）
When gel has set, pour off the butanol and rinse with deionized water.
Pour the stacking gel （~5 ml） and insert the comb immediay.
When the stacking gel has set, place in gel rig and immerse in buffer.
Prior to running the gel, flush the wells out thoroughly with running buffer.

D. Running the gel

After flash spinning the samples, load into the wells.
Be sure to use markers.
We use 15 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly.
Run with constant current （35 - 37 mA with voltage set at > 300 V）.
Usual running time is about 2.5 hr.

E. Using precast gels （Ready Gels from Bio-Rad）:

Assemble gel in gel rig.
Prepare protein samples （10 µg will suffice）.
Use 5 µl of Kaleidoscope standard.
Run at 200 V （constant voltage） for 30 min.

F. Preparation of membrane

Cut a piece of PVDF membrane （Millipore Immobion-P #*H 000 10）.
Wet for about 30 min in methanol on a rocker at room temp.
Remove methanol and add 1x Blotting buffer until ready to use.

G. Membrane transfer