技术中心

牛牛天天人人综合影院 血管生成素样蛋白4(ANGPTL4)检测试剂盒说明书

2015年05月05日 16:24:04人气:979来源:上海沪震生物科技有限公司

资料类型 doc文件 资料大小 20992
下载次数 164 资料图片 【点击查看】
上 传 人 上海沪震生物科技有限公司 需要积分 0
关 键 词 血管生成素样蛋白4(ANGPTL4)检测试剂盒,血管生成素样蛋白4(ANGPTL4)检测试剂盒,血管
【资料简介】

血管生成素样蛋白4(ANGPTL4)检测试剂盒

适用生物 Homo sapiens (Human,人)
血管生成素样蛋白4(ANGPTL4)检测试剂盒检测范围 0.781-50ng/mL 灵敏度 0.29ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 血管生成素样蛋白4(ANGPTL4)检测试剂盒规格 96T


血管生成素样蛋白4(ANGPTL4)检测试剂盒 ELISA Kit for Angiopoietin Like Protein 4 (ANGPTL4)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species Homo sapiens (Human)
Product No. SEB019Hu
Sample type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format 96-well strip plate
Assay length 4.5 hours
Detection range 0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.29ng/mL.

Specificity
This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 4 (ANGPTL4).
No significant cross-reactivity or interference between Angiopoietin Like Protein 4 (ANGPTL4) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Angiopoietin Like Protein 4 (ANGPTL4) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiopoietin Like Protein 4 (ANGPTL4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-98 87
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 81-95 89

Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin Like Protein 4 (ANGPTL4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 79-92% 78-104% 91-99%
EDTA plasma(n=5) 91-98% 95-104% 94-104% 85-102%
heparin plasma(n=5) 94-101% 96-104% 83-93% 79-89%

Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B 1×120μL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediay.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 4 (ANGPTL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 4 (ANGPTL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

上海沪震生物科技有限公司作者

上一篇:elisa实验用的试剂

下一篇:总结ELISA实验操作中常见问题有哪些


我要投稿
  • 投稿请发送邮件至:(邮件标题请备注“投稿”)[email protected]
  • 联系电话0571-87759680
环保行业“互联网+”服务平台
环保在线APP

功能丰富 实时交流

环保在线小程序

订阅获取更多服务

微信公众号

关注我们

抖音

环保在线网

抖音号:hbzhan

打开抖音 搜索页扫一扫

视频号

环保在线

公众号:环保在线

打开微信扫码关注视频号

快手

环保在线

快手ID:2537047074

打开快手 扫一扫关注
意见反馈